Detection of mature embryo sac of esd1 mutants and related RT-qPCR. A, Normal embryo sac of 9522; AP, antipodal cells; PN, polar nuclei; EA, egg cell; Bar = 50 μm. B, Abnormal embryo sac of esd1-m1; Bar = 50 μm. C, Abnormal embryo sac of esd1-m2; Bar = 50 μm. D, Abnormal embryo sac ratio of esd1 mutants. E, Expression of OsAPC6 in the young panicles of 9522 and esd1 mutants. Error bars indicate sd (n = 2); Asterisks indicate significant difference by Student’s t test (^**P < 0.01).