Protein subcellular localization of ESD1. A, Verification of ESD1 transcription activation; SD/-Trp, Tryptophan single-deficient medium; SD/-Trp-His-Ade, Tryptophan, Histidine, and Adenine triple-deficient medium; pGBK-Negative, negative control; pGBK-Postive, positive control. B, Subcellular localization of ESD1 in rice protoplasts; p1305-GFP, the GFP individual expression; p1305-ESD1-GFP, the ESD1 and GFP fusion expression; Bars = 5 μm.