Construction of high-throughput genome-editing libraries of animals. The constructed gRNA libraries are packaged together into the lentiviral vectors, forming a lentiviral library. The lentivirus library is subsequently used to transfect cell lines that can stably express the Cas9 protein. On this basis, positive or negative selection approaches are used to screen cells of interest. With positive screening, only a few cells with target phenotypes can survive and achieve the purpose of gene enrichment. Enriched gRNA-coding fragments are PCR-amplified, and they are subsequently decoded through deep sequencing and data analysis to screen the drug resistance and therapeutic targets. Whereas cells that survive in the negative screening are not the target phenotype cells. It is therefore necessary to compare the abundance of gRNA at different periods to obtain the missing gRNAs by deep sequencing and data analysis, which can then be used to study cell growth and search for tumor-related genes.