Lattice LSFM of transgenic Arabidopsis plants carrying diverse genetically encoded molecular markers which are developmentally and subcellularly regulated in bulging trichoblasts and in outgrowing root hairs. A, ANN1-GFP as a cytosolic marker, (B, Supplemental Video S5) GFP-RHD2 as endosomal and apical PM marker and (C) GFP-FABD2 as actin cytoskeleton marker. Bar = 10 µm (A–C). Imaging: W Plan-Apochromat 25×/1.1 NA, excitation at 488 nm at 75% relative laser power, detection with BP520/30 (a bandpass filter transmitting an emission from 505 to 535 nm), the pixel size 104 nm/pixel, z-stacks of 0.5 µm/plane, encompassing the volume of ∼50.5 µm, exposure time 50 ms, and full stack acquisition 5.3 s.