Biochemical analysis of the brains of Tau 3R/4R mice after unilateral injection of the sarkosyl-insoluble fraction of the tauopathies or control brains into the striatum. Sarkosyl-insoluble fractions were prepared 8 months after injection and analysed by western blotting with anti-tau antibodies T46 (A), AT8 (B), pS396 (C), RD3 (D) and anti-4R tau (E). The red arrowhead in (D) indicates 3R tau and the blue arrowhead in (E) indicates 4R tau. AC = age-matched control; AD = Alzheimer’s disease; CBD = corticobasal degeneration; HD = Huntington’s disease; PiD = Pick’s disease. Molecular weight markers are shown on the left (kDa).