AT8-positive staining after unilateral injection of the sarkosyl-insoluble fraction of patients with corticobasal degeneration (CBD) into the striatum of Tau 3R/4R mice. (A) AT8 staining at 3, 6 and 9 months (M) post-injection. Higher magnification of the boxed area is shown. The sections were counterstained with Kernechtrot stain solution. The scale bar applies to all photomicrographs (25 μm). (B) AT8 staining at 9 months post-injection. Amy = amygdala; Au = auditory cortex; CC = corpus callosum; CG = cingulate gyrus; Ins = insular cortex; M1 = primary motor cortex; M2 = secondary motor cortex; Piri = piriform cortex; PSC = primary sensory cortex; PVC = primary visual cortex; SN = substantia nigra; Stria t = striata terminalis; Thal = thalamus. The scale bar applies to all photomicrographs (50 μm). (C) Astrocytic tau inclusions in Tau 3R/4R mice at 9 months after intracerebral injection of the sarkosyl-insoluble fraction of CBD brain. Sections were stained with AT8 and counterstained with hematoxylin (CBD-injected Tau 3R/4R) or Kernechtrot staining solution (Human CBD). Scale bar = 50 μm.