Analysis of the expression of eugenol-related genes and eugenol contents in stable FaMYB63 transgenic strawberry fruits. RT-qPCR analysis of MYB63, EGS1, CAD1, EGS2, EOBII, and MYB10 transcript levels (A) and GC–MS quantification of eugenol (B) in stable FaMYB63 transgenic strawberry plants. WT, G1 stage fruits of diploid wild strawberry (F. vesca) ‘Ruegen’; FaMYB63-RNAiS1, FaMYB63-RNAiS2, and FaMYB63-RNAiS3 represent G1 stage fruits from three independent FaMYB63-silenced transgenic strawberry lines, respectively. The expression levels of the different genes studied in stable FaMYB63 transgenic strawberry ‘Ruegen’ were expressed as a percentage against their expression levels in WT strawberry plants. Error bars indicate the standard deviation of three biological replicates for each line. Statistical significance was determined using Student’s t test. ***P < 0.001.