FaMYB63 regulates the transcription of FaEGS1, FaEGS2, and FaCAD1. A, Schematic diagrams of the effector (35S:FaMYB63) and reporter vectors (FaEGS1, FaEGS2, and FaCAD1 promoters fused to GUS) that were used for transient expression analysis, GUS staining, and GUS activity assays in transgenic N. benthamiana leaves. pFaEGS1:GUS, pFaEGS2:GUS, or pFaCAD1:GUS with or without the 35S:FaMYB63 effector were co-transformed into N. benthamiana leaves. The blue color indicates activated GUS activity. Data are means ± sd of nine biological replicates. B, Reporter and effector constructs used in the dual-LUC assay. LUC activity analysis, using 35S:FaMYB63 as the effector and P_EGS1:LUC, P_EGS2:LUC, or P_CAD1:LUC as reporters. Promoter activities of FaEGS1, FaEGS2, and FaCAD1 genes activated by FaMYB63 in dual-LUC assays were expressed as the ratio of LUC to REN in N. benthamiana leaves co-transformed with the effector and the reporter combinations. Data are means ± sd of nine biological replicates. Statistical significance was determined using Student’s t test: ***P < 0.001.