Verification of the correlation between 13 miRNAs and 12 target genes using a dual-luciferase reporter assay. A–H, The luciferase activity of the miRNA mimics + mRNA-WT group was lower than that of the NC mimics + mRNA-WT group (P < 0.01), while no significant difference was observed between two MUT groups. I and J, Significant difference of the luciferase activity was also observed between the MUT groups in addition to the WT groups (P < 0.01). Thirteen miRNAs were as follows: ped-miR171a, novel_44 and novel_47 of the MiR171_1 family, ped-miR319b-3p of the MIR159_1 family, ped-miR166a-3p, ped-miR444c, ped-miR169g, ped-miR396d-5p, ped-miR172a-3p, ped-miR164b, ped-miR167c-5p, and ped-miR160a-5p. Twelve target genes were as follows: PeSCL27 (PH02Gene47151), PePCF6_1–2 (PH02Gene09639 and PH02Gene36822), PePAHAL_1–2 (PH02Gene17565 and PH02Gene15869), PeHOX32 (PH02Gene31932), PeMADS27 (PH02Gene21974), PeNF-YA4 (PH02Gene45106), PeGRF6 (PH02Gene06203), PeOsI (PH02Gene07682), PeARF17 (PH02Gene03160), and PeARF18 (PH02Gene37045). Error bars indicate sd of three technical repeats. Statistical analyses were performed by Student’s t test. *P < 0.05; **P < 0.01; ***P < 0.001.