Gene genealogy reconstructed by the Neighbor-Joining method (Saitou and Nei 1987) from variation at the Acph-1 gene region in 99 D. subobscura lines using D. guanche as the outgroup. Genetic distances were estimated by the 2-parameter method (Kimura 1980) with the complete deletion option. Black squares indicate O_st lines. Open, gray and black circles correspond to O₃₊₄, O_3+4+8, and O₃₊₄₊₂₃ lines, respectively