Number of P{hobo, white⁺} insertion sites in the transgenic lines. Southern blot hybridized using the 12.8 probe that reveals the endogenous white gene and the miniwhite gene of the P{hobo, white⁺} insert. yw: empty of hobo host strain for the microinjections; lane α to χ: three independent transgenic lines with one insertion site of the P{three-TPE hobo, white⁺}; lane δ: one transgenic line harboring two insertion sites of the P{three-TPE hobo, white⁺}; lane κ: one transgenic line harboring one insertion site of the P{five-TPE hobo, white⁺}