The TAZ domain and the CaMBD are required for BT2 function and 35S enhancer activity. Suppression of activation-tagged phenotypes by (A) overexpression of TAZ_BT2, and (B) overexpression of BT2_mut in the yucca1d line. C, YUCCA1 expression in yucca1d, yucca1d 35S:TAZ_BT2, and yucca1d 35S:BT2_mut lines. Total RNA was extracted and subjected to RT-qPCR. EIF-4A2 was used to normalize the expression data. Expression values are plotted relative to the WT line (set to ∼1). Error bars represent se among biological replicates (n = 3). The asterisks represent statistically significant differences between the indicated samples and the yucca1d line as determined by two-sample t test (P ≤ 0.01).