Short hairpin RNAs (shRNAs) against hits from the genome-wide association study affect the embryo resistance rate. Each blue point represents the embryo resistance rate ± SE in a single shRNA line, and black points represent control lines. The dashed line separates two sets of shRNA lines that each correspond to a different control line. The solid line separates lines analyzed for full embryo r2 resistance and mosaic resistance. * P < 0.05, ** P < 0.01, *** P < 0.001, Fisher’s exact test, comparing with the corresponding control line. After multiple testing correction, only the points marked ** or *** were considered significant.