HIF1α promotes the proliferation and migration of PTC cells by increasing the expression of miR-146a. (A) Expression of miR-146a-5p and miR-146a-3p in PTC tissues and normal thyroid tissues based on microarray GSE113629, abscissa indicates sample type. (B) Expression of miR-146a in clinical PTC tissues and adjacent normal tissues (n = 45) determined by RT-qPCR. (C) Correlation between miR-146a and LSD1 determined by Pearson. (D) Enrichment of HIF1α in the promoter region of miR-146a detected by RT-qPCR after ChIP. (E) Expression of HIF1α and miR-146a in K1 cells after transfection with oe-HIF1α determined by RT-qPCR. (F) Expression of HIF1αin K1 cells after transfection with oe-HIF1α determined by Western blot analysis. (G) EdU labeling showing proliferation of K1 cells after inhibition of both HIF1α and miR-146a or either alone (× 200). (H) Scratch test showing migration of K1 cells after inhibition of both HIF1α and miR-146a or either alone. (I) Transwell assay displaying invasion of K1 cells inhibition of both HIF1α and miR-146a or either alone (× 200). (J) TUNEL detection displaying apoptosis of K1 cells after inhibition of both HIF1α and miR-146a or either alone (× 200). (K) Western blot analyses of expression of EMT-related proteins E-cadherin, β-catenin, N-cadherin, and Vimentin in K1 cells after inhibition of both HIF1α and miR-146a or either alone. The data in the figure were all measurement data, which were expressed by the mean ± standard deviation. The data between cancer and adjacent normal tissues in panel B did not conform to the normal distribution and were analyzed using Wilcoxon matched-pairs signed rank test, and unpaired data between 2 groups were analyzed by unpaired t test. Multigroup comparison was conducted using one-way ANOVA. The experiment was independently repeated 3 times. *P < 0.05.