(A) Number of cytoquakes detected per cell per hour and (B) number of cytoquakes detected per post pair per hour in 3T3 fibroblasts on different substrate stiffnesses and for different cell types, where CF = cardiac fibroblast and CMF = cardiac myofibroblast. Each datapoint represents the detected cytoquake rate within an individual cell. Horizontal lines are average values (±S.E). The variations in B between different values of k were significant via a one-way ANOVA test over the full dataset (P < 0.001) (^*: P < 0.05). (C and D) Scatter plots showing anticorrelation between number of cytoquakes detected per post pair per hour and (C) total force per cell and (D) cell spread area (number of associated posts) for 3T3 fibroblast cells. The data in C and D are color coded for different substrate stiffnesses. Each datapoint represents an individual cell.