Immunodetection of SPACA1, ZPBP, TPPP2 and SLIT2 in the seminiferous tubules of Cfap65^+/+ and Cfap65^−/− mouse testes during different stages. (A) Compared with Cfap65^+/+ testes, a reduced and irregular expression of SPACA1 at steps 5–6 (stages V–VI) and steps 11–12 (stages XI–XII) spermatids in Cfap65^−/− testes were shown. (B) Compared with Cfap65^+/+ testes, a reduced expression of ZPBP at steps 5–6 (stages V–VI) and steps 11–12 (stages XI–XII) spermatids in Cfap65^−/− testes were shown. (C) Compared with Cfap65^+/+ testes, a reduced expression of TPPP2 in sperm MS at steps 15–16 (stages VI–VIII) and in the spermatid cytoplasm at steps 11–12 (stages XI–XII) in Cfap65^−/− testes was shown. (D) SLIT2 staining of seminiferous tubules from Cfap65^+/+ and Cfap65^−/− mouse testes. A failure of SLIT2 assembly in the sperm flagella at step 15 (stages VI–VII) spermatids and an abnormal expressional pattern at steps 11–12 (stages XI–XII) spermatids in Cfap65^−/− testes is shown. Scale bars: 5 μm.