qPCR validation of whole-genome sequencing mtDNAcn estimates. MtDNAcn estimates from 95 randomly selected whole-genome sequenced samples are plotted along the x-axis vs mtDNAcn estimates derived from qPCR of the same samples plotted along the y-axis. Pearson correlation test P value is 2-sided. mtDNAcn = mitochondrial DNA copy number; qPCR = quantitative polymerase chain reaction; qRT-PCR = real-time quantitative PCR.