In vitro translated PCV 1 RdRp is active as an RT. (A) a schematic of in vitro translation of PCV1 RdRp and determination the RT activity of protein through RT-PCR. (B) RT-PCR analysis using PCV1 dsRNA as RNA template and PCV1-RdRp primers; M, DNA marker, 1, in vitro translated PCV1 RdRp as enzyme, 2, negative control (no enzyme), 3, negative control (no primer), 4, MMuLV used as enzyme. (C) RT-PCR analysis using PCV1 dsRNA as RNA template and PCV1-RdRp primers, RT reactions at 42°C; M, DNA marker, 1, negative control (no enzyme), 2, in vitro translated PCV1 RdRp used as RT enzyme, 3, MMuLV used as enzyme.