Estimation of CO distribution in WT lettuce and Lsfancm. A, Distribution of chiasmata per chromosome pair during metaphase I. The chiasmata distribution in WT deviates significantly from Lsfancm-1 and Lsfancm-2. WT vs Lsfancm-1, P = 4.74E-11; WT vs Lsfancm-2, P = 4.11E-11, Chi-square test. B, Statistical analysis of the number of LsHEI10 foci at pachytene in WT and Lsfancm-1 and Lsfancm-2. n.s, not significant, two-tailed Mann–Whitney test. C, Immunostaining of LsHEI10 in WT, Lsfancm-1, and Lsfancm-2 at zygotene and pachytene. DAPI signals are shown in blue and LsHEI10 foci are shown in red. Bars = 5 µm.