SYP31 and SYP32 colocalize with COG3 and are essential for the Golgi localization of COG3. A, SYP31 or SYP32 colocalize with COG3 when transiently expressed in N. benthamiana leaf epidermal cells. The linear Pearson correlation coefficient (r_p) between mCherry-SYP3 and COG3-GFP was obtained using ImageJ with the PSC colocalization plug-in. B, Loss of COG3 Golgi signal in ∼22% of pollen from syp31/+ syp32-1/+ expressing pLAT52:COG3-GFP (n = 128). C, γ-COP localization in ∼24% of pollen from the syp31/+ syp32-1/+ mutant expressing pLAT52:γ-COP- mCherry (n = 83). D, Confocal images of WT and cog3 MP expressing pSYP31:mCherry-SYP31 and pSYP32:mCherry-SYP32. BCP was examined in (B and C). Boxed regions were selected at random and enlarged in the right-hand panels (A) or lower panels (B and C). Bars = 10 μm in (A), 5 μm in (B, C, and D).