Figure 3.
![Functional and kinetic characterization of CsSWEET7a in yeast. A, Concentration-dependent [14C]Glucose uptake rate. FW, fresh weight. B, Kinetic analysis of CsSWEET7a. The Km was 10.0 ± 0.8 mM. C, Substrate specificity of CsSWEET7a expressed in EBY.VW4000. Substrate specificity was determined by competitive inhibition of [14C]Glucose uptake. The sample without competitor was the control, the concentration ratio of the competing sugar relative to that of glucose was 10. The values are presented as units relative to the values from the control, taken as 100%. Mean values ± SE of three independent biological replicates are given. Significance compared to control was determined by Student’s t test at *P < 0.05, **P < 0.01, and ***P < 0.001.](https://oup.silverchair-cdn.com/oup/backfile/Content_public/Journal/plphys/186/1/10.1093_plphys_kiab046/2/kiab046f3.jpeg?Expires=1750482782&Signature=o83uJy926jz7ea04OdwFGjXCz3p304cNkZzSxeTcRJJ-Sj96YS96J-kf0waM99HXWFVe0VBvPqnthPRDoRXBUdTD~N75i-Nokm5PHxcfj-T4Mq0jbAzTWlELgkGHhwsqIUACX54-vxzl~UQW0dhdIN1mx0xQFnhFP9tmdi3tOtv0iFz6zuuwj94-NGFMbUMu8MZWk5m4rnYBEtRMoj~WeTuoI2cdOUcx38L8HrjeWoFSpVJdiQV6hv-5C2ZyOK82FkeKgqm5s3ZiE9f83bRnVn~pXeGx9ZZtZ-SMhDcbgCMjEcL4YBAQD6Ukto3Lf0b7I82fNlSgeMSBzeIPIGp1jw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Functional and kinetic characterization of CsSWEET7a in yeast. A, Concentration-dependent [14C]Glucose uptake rate. FW, fresh weight. B, Kinetic analysis of CsSWEET7a. The Km was 10.0 ± 0.8 mM. C, Substrate specificity of CsSWEET7a expressed in EBY.VW4000. Substrate specificity was determined by competitive inhibition of [14C]Glucose uptake. The sample without competitor was the control, the concentration ratio of the competing sugar relative to that of glucose was 10. The values are presented as units relative to the values from the control, taken as 100%. Mean values ± SE of three independent biological replicates are given. Significance compared to control was determined by Student’s t test at *P < 0.05, **P < 0.01, and ***P < 0.001.
