Uptake of pH Rodo-labelled antibodies by monocyte-derived dendritic cells.

[A] Three different glyco-forms of anti-TNF-alpha antibodies were used: Fuc-G0 with fucose and GlcNAc, Fuc-G2S1/G2S2 with fucose and sialic acid, and G2S1/G2S2 with sialic acid as a main N-linked glycosylation modification. [B] Uptake of pHrodo labelled anti-TNF-alpha antibodies by monocyte-derived dendritic cells was measured as mean fluorescence intensity [n = 4]. To investigate the effect of LPS stimulation and FcR blocking on antibody uptake, four experimental conditions were tested [no FcR blocking and no LPS stimulation, no FcR blocking and LPS stimulation, FcR blocking and no LPS stimulation, FcR blocking and LPS stimulation].