The spectrum of different brain damage severity outcomes after the same duration of HI determined using histopathology and MRI. (A) Different degrees of brain damage outcomes, namely mild, moderate and severe, were observed using the T2-weighted images (upper panel), Nissl-stained histopathology (lower panel) and gross anatomy (right panel) 7 days after the same duration of HI. (B) The ipsilateral cerebral hemispheric volume loss compared with the contralateral cerebral hemisphere by using histopathology was used to represent the spectrum of brain damage severity outcomes after HI. N = 24 for histopathology. (C) A Raster plot of brain damage severity outcome in the relationship between the percentages of hemispheric volume reduction measured by MRI and that by histopathology (IHC). The linear regression line was plotted as the overall regression was significant (P < 0.001) determined by using a Spearman’s correlation test. The inset shows the exclusion of the oedema formation region during the calculation of MR-derived brain damage outcomes based on T2-weighted images. Light blue, ipsilateral hemisphere; dark blue, contralateral hemisphere. N = 20 for MRI.
