Identification of candidate aging/heathy aging-related lncRNAs (car-lncs). (A) Workflow of analyzing lncRNAs in 171 individuals from longevous families; (B) Venny diagram of DElncRNAs (FDR < 0.1) among different groups and regions (‘N-Hn CEN_vs_F1SP_ DElnc’ means DElncRNAs between CENs and F1SP in N-Hn region, and so on). F1SP as the controls were compared separately with F1s and CENs to get DElncRNAs. In N-Hn region, nine lncRNAs are dysregulated in both F1 and CEN comparing with F1SP group; using the same method, seven dysregulated lncRNAs were found in S-Hn individuals, of which one lncRNA is overlapped with N-Hn’s; (C and D) Volcano plot of lncRNAs expression by comparing CEN and F1SP in N-Hn (C) and S-Hn (D): blue spots are SDElncRNAs; (E) Venny diagram of SDElncRNAs (FDR < 0.05) between CEN and F1SP in N-Hn and S-Hn regions: 61 SDElncRNAs were identified in N-Hn samples and 41 in S-Hn, among which 7 lncRNAs are overlapped; (F) Heatmap was plotted to visualize the expression of 7 overlapped SDElncRNAs in the blood samples of individuals: 100 randomly selected individuals (the number that ahead of ‘_CEN/F1/F1SP’ is a serial number for each sample; ‘F’ and ‘M’ means female and male, respectively). The deeper red indicates higher expression, while the deeper blue indicates lower expression levels.