Impact of Sirt5 overexpression on photochemical-induced arterial thrombosis in vivo in mice. (A) Sirt5^Tg/0 mice showed reduced time to formation of an occlusive thrombus in their carotid arteries after endothelial-specific damage as compared to Sirt5^+/+ littermates. (B) Representative trace of mean blood flow until occlusion (mean flow ≤ 0.1 mL for 1 min) in the two study groups. (C) Sirt5 overexpression reduced the number of episodes of thrombus embolization. (D–F) No difference in terms of initial heart rate, blood flow, and weight were reported among Sirt5^Tg/0 and wild-type littermates. n = 7–8 different mice per group. (A and C–F): unpaired two-tailed Student’s t-test. *P < 0.05; ***P < 0.001.