Scan of selective signals in the domestic Italian breed Camosciata. (A) The PBS statistic was constructed using the breed Camosciata as target, the breed Selvatica as control and the grouped wild HGF background. (B) The Pi-Ratio was calculated from the ratio of nucleotide diversity for the grouped wild HGF background to that for the breed Camosciata. All dots represent the sliding window of 10 kb with 10 kb step size. The windows with values over top 1% quantile for both two statistics were overlapped to identify selective genes which were noted with cross labels. (C) The genes located in the LD block of scaffold 1:7,845,520–17,375,296. The selective genes were in red. (D) The Camosciata breed with cream white plumage (mutation) and the Selvatica breed with wild plumage type. The Camosciata was selected from a small breeding flock originated in France and brought to Italy in 1922 and differs from the solid white variety due to the pigmented skin of the neck and the visible white spotting of the plumage absent in the solid white birds. The photos were taken at Az. Agricola E. Oggioni in Italy and provided by Maria Giuseppina Strillacci and Erica Gorla. (E) The mutation p.Trp218Gly in exon 1 of TYR gene. The alignment of protein sequences was shown. This mutation was indicated by star label. The neighboring histidine residues which were essential for TYR function via binding to copper ions were noted with arrows.