Diphtheria toxin-mediated ablation of Na_v1.8-positive nociceptors decreases oxaliplatin-induced cold allodynia. (A) Cartoon of diphtheria toxin-mediated ablation of Na_v1.8-positive neurons. [B(i)] Histogram of cross-sectional areas of all cold-sensing neurons imaged in Na_v1.8-Cre DTA mice treated with oxaliplatin. [B(ii)] Cumulative probability plot of cell areas in oxaliplatin-treated Na_v1.8-Cre (blue) and Na_v1.8-Cre DTA (red) mice, compared by Kolmogorov-Smirnov test. The distribution of cell areas in vehicle-treated Na_v1.8-Cre mice is shown for comparison. n = 108 cells from six oxaliplatin-treated Na_v1.8-Cre mice (four males and two females), n = 46 cells from two oxaliplatin-treated Na_v1.8-Cre DTA mice (one male and one female) and n = 14 cells from four vehicle-treated Na_v1.8-Cre mice (two males and two females). (C) Quantification of the number of nociceptive behaviours in 5 min on the 5°C cold plate in 10 control and 8 Na_v1.8-Cre DTA mice treated with oxaliplatin.