Schematic representation of other erm(B)-containing structures. (a) Representation of the elements found in six strains that harbour only erm(B). This structure is similar to that found in the GAS ICE HKU372 (Run SRA accession number ERR060322).⁷ (b) Structure found in GAS748HUB that is very similar to Tn6263 previously described in S. gallolyticus NTS31106099 (accession number JYKU01000013)⁴⁸ harbouring erm(B) and aminoglycoside-modifying enzymes. The regions containing partial Tn916 are underlined with a green dotted line. The grey-shaded areas connect regions based on similarity (over 98% identity). Double slash (\\) represents sequence interruption by different contigs. Genes are represented with arrows; the colours represent different genes. Grey, GAS reference genes (M1GAS, accession number NC_002737). Resistance genes: erm(B) in red and aminoglycoside-modifying enzymes in orange. Light blue, genes harboured in Tn916-like elements. Yellow, genes related to integration/excision module (integrases, recombinases, excisionases and transposases). Blue, mobilization module genes (T4SS genes and relaxases). Dark green, replication module genes (topoisomerases, regulatory proteins and helicases). Representative isolates are highlighted in boldface and the other isolates with the same structure in grey; under the name is showed the emm/ST type of each isolate. This figure appears in colour in the online version of JAC and in black and white in the print version of JAC.