Figure 3.
Analysis of the genetic origin and BMAL1 expression of cloned monkeys. (A–E) Examples of SNPs for B1–B5, respectively, showing mtDNA SNPs that were identical to those of the oocyte donor monkey, but different from those of surrogate monkey and donor fibroblasts. (F) Examples of STRs from ear tissue samples taken from five cloned monkeys (B1–B5), showing that their nuclear DNA was identical to that of the donor fibroblast, but different from those of their oocyte donor and surrogate monkeys. A more extensive list of the STRs is given in Table S2. (G) Reverse transcription PCR analysis of BMAL1 expression on the blood sample from cloned monkey B1 showed a complete absence of the wild-type BMAL1 transcript.

Analysis of the genetic origin and BMAL1 expression of cloned monkeys. (A–E) Examples of SNPs for B1–B5, respectively, showing mtDNA SNPs that were identical to those of the oocyte donor monkey, but different from those of surrogate monkey and donor fibroblasts. (F) Examples of STRs from ear tissue samples taken from five cloned monkeys (B1–B5), showing that their nuclear DNA was identical to that of the donor fibroblast, but different from those of their oocyte donor and surrogate monkeys. A more extensive list of the STRs is given in Table S2. (G) Reverse transcription PCR analysis of BMAL1 expression on the blood sample from cloned monkey B1 showed a complete absence of the wild-type BMAL1 transcript.

Close
This Feature Is Available To Subscribers Only

Sign In or Create an Account

Close

This PDF is available to Subscribers Only

View Article Abstract & Purchase Options

For full access to this pdf, sign in to an existing account, or purchase an annual subscription.

Close