Identification of recombinant PRMT7-knockdown lentivirus in the developmentally arrested embryos (A) PRMT7 knockdown in the developmentally arrested embryos. a,a’: control group (no injection); b,b’: pLKO.1-EGFP lentivirus was injected into the developmentally arrested embryos; c,c’: pLKO.1-EGFP-shhPRMT7 lentivirus was injected into the developmentally arrested embryos; d,d’: control group (no injection, stained by PRMT7 antibody); e,e’: pLKO.1-EGFP lentivirus was injected into the developmentally arrested embryos (injection, stained by PRMT7 antibody); f,f’: pLKO.1-EGFP-shhPRMT7 lentivirus was injected into the developmentally arrested embryos (injection, stained by PRMT7 antibody). All the experiments were repeated three times. Scale bar: 50 μm. (B) Quantitative comparison of the PRMT7 protein levels in human IVF embryos of the control group (no injection), injection of pLKO.1-EGFP lentivirus group and injection of pLKO.1-EGFP-shhPRMT7 lentivirus group. Data are presented as the mean ±SD. ***P < 0.001.