The expression level of BAX and CASP3 mRNA in different treatment groups. The relative abundance of BAX (a) and CASPS3 (b) mRNA was measured in a real-time qPCR platform where GAPDH was used as the housekeeping gene. The result showed that the expression of both genes was significantly increased in 200 and 500 mM concentrations compared with the untreated control group. Statistically significant effects (P ≤ 0.05) of different treatments on gene expression are marked with asterisks, where ^* = significantly different in different ethanol treatments compared with the control group and ^# = significantly different in two time points.