Plasmid carriage by genotype 11-1010 over time. Examination of plasmid pCM410 retention/loss over time was examined in strains of Methylorubrum extorquens AM1 containing evolved mutations identified in a previous study (Chou et al. 2011). Plasmid carriage by this strain was examined in biological replicate of N = 20. Lines indicate the change of each individual lineage. Experiments were carried out in Hypho media with relaxed selection for the plasmid (no antibiotic present). The dashed gray line in each panel indicates the experimental limit of detection for the assay.