PR-induced nuclear accumulation of Staufen is mediated by TBPH. (A) A subcellular localization of Staufen-GFP upon knockdown of TBPH in C4da neurons (Genotypes. CTRL: UAS-Stau-GFP/+; PPK^1a-gal4 > UAS-mCD8RFP/UAS-Luciferase, TBPH RNAi: UAS-Stau-GFP/+; PPK^1a-gal4 > UAS-mCD8RFP/UAS-TBPH RNAi). Arrow indicates the increased nuclear accumulation of Staufen-GFP. Scale bar (yellow), 2 μm; (white), 10 μm. (B) Quantification of the amount of Staufen-GFP normalized to controls. ^****P < 1.0|$\times$|10⁻⁴ by paired two-tailed student’s t-test; error bars, ± SEM; n ≥ 57 Staufen-GFP puncta; n = 3 larvae. (C) A subcellular localization of Staufen-GFP upon knockdown or overexpression of TBPH in combination with V5-PR36 expression in C4da neurons (Genotypes. CTRL: UAS-Stau-GFP/+; PPK^1a-gal4 > UAS-V5-PR36, UAS-mCD8RFP/UAS-Luciferase, TBPH RNAi: UAS-Stau-GFP/+; PPK^1a-gal4 > UAS-V5-PR36, UAS-mCD8RFP/UAS-TBPH RNAi, TBPH OE: UAS-Stau-GFP/UAS-TBPH OE; PPK^1a-gal4 > UAS-V5-PR36, UAS-mCD8RFP/+). Scale bar (white), 10 μm. (D) Quantification of the amount of Staufen-GFP normalized to controls. ns: not significant; ^*P = 0.0248 by paired two-tailed student’s t-test; error bars, ± SEM; n ≥ 17 Staufen-GFP puncta; n = 3 larvae.