Exportin- and Importin-dependent changes of nuclear Staufen-GFP upon V5-PR36 expression. (A) A subcellular distribution of Staufen-GFP in C4da neurons with or without Exportin 5 knockdown. Merge: merged with plasma membrane marker (Genotypes. CTRL: UAS-stau-GFP/+; PPK^1a-Gal4,UAS-mCD8RFP/UAS-luciferase, Exportin 5 RNAi: UAS-stau-GFP/+; PPK^1a-Gal4,UAS-mCD8RFP/UAS-Exportin 5 RNAi). Dashed circular lines (white) outline the nuclei. Scale bar (white), 10 μm. (B) The pixel number and the mean intensity of nuclear Staufen-GFP puncta were multiplied to provide an estimate of the amount of nuclear Staufen-GFP. ns: not significant by two-tailed student’s t-test; error bars, ± SEM; n ≥ 24 Staufen-GFP puncta; n = 3 larvae. (C) A subcellular distribution of Staufen-GFP in C4da neurons expressing V5-PR36 with or without knockdown of Exportin 5 (Genotypes. CTRL: UAS-stau-GFP/+; PPK^1a-Gal4,UAS-mCD8RFP, UAS-V5-PR36/UAS-luciferase, Exportin 5 RNAi: UAS-stau-GFP/+;PPK^1a-Gal4,UAS-mCD8RFP, UAS-V5-PR36/UAS-Exportin 5 RNAi). Arrow (white) indicates increased nuclear Staufen-GFP puncta. Scale bar (white), 10 μm. (D) The pixel number and the mean intensity of nuclear Staufen-GFP puncta were multiplied to provide an estimate of the amount of nuclear Staufen-GFP. Arrow (white) indicates conjunction of nuclear-accumulated Staufen-GFP. Dashed circular lines (white) outline the nuclei. Scale bar (white), 10 μm. ^**P = 0.0071 by two-tailed student’s t-test; error bars, ± SEM; n ≥ 29 Staufen-GFP puncta; n = 3 larvae. (E) Quantification of the amount of nuclear Staufen-GFP per puncta in C4da neurons expressing the denoted transgenes (see Fig. S4A for details). ^*P < 0.05; ^**P < 0.01; ^***P < 0.001 by two-tailed student’s t-test; error bars, ±SEM; n ≥ 9 Staufen-GFP puncta; n = 3 larvae.