NLR-Annotator step-by-step. A, A genomic sequence is dissected into 20-kb fragments overlapping by 5 kb. B, Each fragment is translated in all six reading frames. C, Amino acid sequences are screened for NLR-associated motifs (see inset). D, The positions of motifs are projected back onto their originating 20-kb genomic fragments. E, Motif-containing genomic fragments are merged, and redundant motifs resulting from overlaps are removed. F, Motif combinations associated with an NB-ARC domain (inset, right side) are identified in the genomic sequence (stippled boxes). Overlapping combinations are merged. G, The NB-ARC locus is used as a seed to search the DNA sequence upstream and downstream for additional NLR-associated motifs (e.g. those indicative of a coiled coil or LRR). H, The final NLR locus is reported.