Complementation of cepr1 mutants using a CEPR1 genomic fragment. Reproductive development phenotypes were assessed for independent complementation (comp) lines in the No-0 cepr1-1 (1 and 2) and Col-0 cepr1-3 (3 and 4) backgrounds compared with their respective cepr1 mutant and wild-type lines. A and B, Representative images showing side views of the inflorescence (top row) and dissected postanthesis flowers (bottom row) for lines in the No-0 (A) and Col-0 (B) ecotypes. C and G, Quantification of ovule number for siliques with nonzero fecundity for No-0 (n = 4–10, two siliques per plant; C) and Col-0 (n = 3–11, one to four siliques per plant; G) ecotype lines. D and H, Measurement of seed set for No-0 (n = 4–10; D) and Col-0 (n = 3–11; H) ecotype lines. Two siliques were examined per plant from positions on the stem corresponding to the phase of wild-type maximal seed set (Fig. 3, C and D). E and I, Frequency of aborted seeds observed at fertilized ovule positions for lines in the No-0 (n = 4–10, two siliques per plant; E), and Col-0 (n = 3–11, one to four siliques per plant; I) ecotypes. F and J, Seed area for No-0 (n = 6–11 plants; F) and Col-0 (n = 3–9 plants; J) ecotype lines determined from 60 to 120 seeds per plant. Significant differences were determined by ANOVA followed by Fisher’s lsd test (α = 0.05). Error bars show se.
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