2-PG content in leaves of Col-0 and two HPR mutants. 2-PG was measured by HPLC/MS-MS. Leaves were harvested after 6 h in 125 or 1,000 μmol photons m−2 s−1. Because the data for both light intensities were indistinguishable, they were combined to increase statistical power. Data are expressed as peak area of 2-PG divided by peak area of the internal standard relative to the weight of plant material extracted. The results in high and low light were not different and so all of the data were combined. Differences between the wild type and treatments were tested by one-way ANOVA followed by Tukey’s test. The box encompasses the middle two quartiles, the mean is shown as an open square inside the box, the median is shown as a line inside the box, and the whiskers show the sd of the data. Statistical differences are indicated as follows: *, α = 0.05. n = 7 or 8.
