Phosphorylation-dependent homomerization of Ehd1. co-IP analyses were performed between Ehd1-HA and Ehd1-Myc after CIP treatment. Vectors were transformed into protoplasts prepared from Oc cells. Before immunoprecipitation with anti-HA antibodies, the protein extracts were incubated with or without CIP for 2 h at 37°C. Immunoprecipitated proteins were separated by SDS-PAGE, and signals were detected by HRP-conjugated anti-Myc antibodies.