Role of AtGLR3.3 in the innate immunity and GSH-triggered defense response in the leaf. A, Leaves of ecotype Columbia (Col-0) and atglr3.3-1 and atglr3.3-2 mutants were infiltrated with either CK solution or the solution containing 100 µm GSH 1 d before the inoculation of Pst DC3000 at 5 × 105 cfu mL–1. The pathogen proliferation was monitored at 0, 1, and 2 DPI. Values presented are average with se from at least four leaves from different plants. At 1, 2, and 3 DPI, there is sd between the Col-0 and two mutants at P < 0.01. Two asterisks indicated significance at P < 0.01. Five independent experiments were conducted and showed comparable results. B, Leaf symptoms of the Col-0, atglr3.3-1, and atglr3.3-2 plants preinfiltrated with CK solution or the solution containing 100 µm GSH 3 DPI with Pst DC3000. C, Same leaves in B were kept 1 d longer, e.g. 4 DPI, in a sealed agar plate.
