Figure 2.
UBP12 and UBP13 have similar expression pattern and protein localization. A to D, GUS staining of dark-grown seedlings (A), 4-d-old seedlings under LD condition (B), 14-d-old seedlings under LD condition (C), and inflorescences (D) of the transformants containing UBP12pro:GUS. E to H, GUS staining of dark-grown seedlings (E), 4-d-old seedlings under LD condition (F), 14-d-old seedlings under LD condition (G), and inflorescences (H) of the transformants containing UBP13pro:GUS. I to L, UBP12 fused to GFP (K) and UBP13 fused to CFP (L) localize to the nuclei and cytoplasm; 35Spro:GFP (I) and 35Spro:CFP (J) were used as control. Bar = 200 µm. UBP12/UBP13 protein was detected in both cytoplasmic and nuclear fractions. Histone H3 and phosphoenolpyruvate carboxylase (PEPC) were used as control for nuclear or cytoplasmic fraction, respectively.

UBP12 and UBP13 have similar expression pattern and protein localization. A to D, GUS staining of dark-grown seedlings (A), 4-d-old seedlings under LD condition (B), 14-d-old seedlings under LD condition (C), and inflorescences (D) of the transformants containing UBP12pro:GUS. E to H, GUS staining of dark-grown seedlings (E), 4-d-old seedlings under LD condition (F), 14-d-old seedlings under LD condition (G), and inflorescences (H) of the transformants containing UBP13pro:GUS. I to L, UBP12 fused to GFP (K) and UBP13 fused to CFP (L) localize to the nuclei and cytoplasm; 35Spro:GFP (I) and 35Spro:CFP (J) were used as control. Bar = 200 µm. UBP12/UBP13 protein was detected in both cytoplasmic and nuclear fractions. Histone H3 and phosphoenolpyruvate carboxylase (PEPC) were used as control for nuclear or cytoplasmic fraction, respectively.

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