Figure 2.
UBP12 and UBP13 have similar expression pattern and protein localization. A to D, GUS staining of dark-grown seedlings (A), 4-d-old seedlings under LD condition (B), 14-d-old seedlings under LD condition (C), and inflorescences (D) of the transformants containing UBP12pro:GUS. E to H, GUS staining of dark-grown seedlings (E), 4-d-old seedlings under LD condition (F), 14-d-old seedlings under LD condition (G), and inflorescences (H) of the transformants containing UBP13pro:GUS. I to L, UBP12 fused to GFP (K) and UBP13 fused to CFP (L) localize to the nuclei and cytoplasm; 35Spro:GFP (I) and 35Spro:CFP (J) were used as control. Bar = 200 µm. UBP12/UBP13 protein was detected in both cytoplasmic and nuclear fractions. Histone H3 and phosphoenolpyruvate carboxylase (PEPC) were used as control for nuclear or cytoplasmic fraction, respectively.