The predicted ARSs compared with the annotated ARSs and experimental datasets related to DNA replication. (A) An example from chromosome XI, from left to right, shown along the chromosome coordinate; the following information is shown from top to bottom: the black vertical rectangle represents the total predicted ARSs and the top-ranked ARS predictions. The green vertical rectangle describes the annotated ARS datasets, including the data from the SGD, DeOri and OriDB (containing confirmed, likely and dubious ARSs) databases, as well as miniARS-seq analysis [21] and plasmid-based assay; the blue plots present the ORC and MCM ChIP signals [35] and replication timing profile [36] of chromosome XI. (B) An example of predicted ARS from chromosome XI, the black line describes the local |${z}^{\prime }$| curve. The plot of |${z}^{\prime }$| curve is also called cumulative GC profile [23]. When A/T bases of local sequence exceed global sequence, the local |${z}^{\prime }$| curve shows a monotonic increase; otherwise, the local |${z}^{\prime }$| curve shows a monotonic decrease. The bright red rectangle represents the predicted ARS, and the dark red rectangle represents the scanned ACS motif. The bright blue rectangle shows the annotated ARS records from the SGD, OriDB and DeOri databases, and the dark blue rectangle represents the annotated ACS motif of ARS1125 according to SGD. The yellow rectangle shows the annotated ARS records from different ARS experimental data, including miniARS-seq analysis and plasmid-based assay.