Sequence feature analysis of replication origins. (A) |$k^{\prime}$| and |${\mathrm{R}}^2$| of the fitted |$\mathrm{z}^{\prime}$| curve of 352 ARSs annotated in the SGD database with both |${k}^{\prime }>0$| (red) and |${k}^{\prime }<0$| (blue). The circle size represents the AT content of each sequence. (B) |$k^{\prime}$| and |${\mathrm{R}}^2$| of the fitted |$\mathrm{z}^{\prime}$| curve of 352 non-ARSs randomly extracted from intergenic sequences of S. cerevisiae S288C with both |${k}^{\prime }>0$| (red) and |${k}^{\prime }<0$| (blue). The circle size represents the AT content of each sequence. (C) Comparison of |$k^{\prime}$|⁠, the slope of the fitted |$\mathrm{z}^{\prime}$| curve, between ARSs and non-ARSs. (D) Comparison of |${\mathrm{R}}^2$|⁠, the coefficient of determination, between ARSs and non-ARSs. (E) Comparison of AT content between ARSs and non-ARSs. Center black line, median; boxes, interquartile range (IQR); whisker, 1.5 × IQR; data points beyond the whiskers are outliers. Significance of the above analysis is estimated by the pairwise Wilcox test, and ‘***’ represents P-value <0.001.