Heatmap showing the fraction of overlap of the clusters between the manually curated OGs (only examples are labelled) and the inferred OGs from, in this case, Orthofinder BLAST as example. The manually curated OGs (y-axis) are sorted from the highest to the lowest overlap with an Orthofinder OG (x-axis), creating a diagonal (dark purple). The nondiagonal part (lower right corner) is clustered with the weighted clustering method. On the left side of the diagonal, there are clear examples of undersplitting of the manually curated OGs. To the right of the diagonal (lower right corner), there are clear examples of oversplitting or misclassifications. The colorbar shows the fraction of overlap between the manual OG sequences and the automated OG sequences.