Loss distributions of LECA OGs estimated from different orthology inference methods. Except for Ancestral Panther, SonicParanoid and SwiftOrtho, the loss distributions show similar patterns. For eggNOG hmm versus Orthofinder BLAST/Broccoli and eggNOG DIAMOND versus Orthofinder DIAMOND, there is no significant difference between distributions (Kruskal–Wallis H test P-value >0.001).