Collagen I gene expression evaluated by qPCR and renal interstitial fibrosis: Masson’s thrichromic staining. SHAM-operated (SHAM); CRF rats + vehicle (CRF + vehicle, Group 1), CRF rats + calcitriol (CRF + 1,25VD, Group 2), CRF rats + paricalcitol (CRF + PARI, Group 3). (A) Collagen I (Col I) mRNA. (B) Representative Western blot of fibronectin and GAPDH and Fibronectin western blot quantification. (C) Masson’s trichrome staining interstitial fibrosis representative photo of Masson’s trichrome staining 10× and quantification in all rats. Data represent median and interquartile range. *P < 0.05 versus CRF + vehicle; ^#P < 0.05 versus CRF + 1,25VD.