TGF-β gene expression evaluated by qPCR and immunohistochemitry. SHAM-operated (SHAM); CRF rats + vehicle (CRF + vehicle, Group 1), CRF rats + calcitriol (CRF + 1,25VD, Group 2), CRF rats + paricalcitol (CRF + PARI, Group 3). (A) TGF-β mRNA. (B) TGF-β immunohistochemistry. (C) Representative photo of TGF-β immunohistochemistry 10×. Data represent median and interquartile range. *P < 0.05 versus CRF + vehicle; ^#P < 0.001 versus CRF + 1,25VD.