Phylogenetic tree and subcellular localization of the MOF1 protein. A, Phylogenetic tree analysis. The phylogenetic tree was performed using the neighbor-joining method, and bootstrap support values calculated from 1000 replicates are given at the branch nodes. At, Arabidopsis thaliana; Bradi, Brachypodium distachyon; Brast, Brachypodium stacei; Glyma, Glycine max; Gorai, Gossypium raimondii; Pavir, Panicum virgatum; Pahal, Panicum hallii; PGSC, Solanum tuberosum; Seita, Setaria italica; Sevir, Setaria viridis; Sobic, Sorghum bicolor; Solyc, Solanum lycopersicum; Zm, Zea mays. B to D, Single GFP protein in rice protoplasts. E to G, MOF1-GFP in rice protoplasts. H to J, Single YFP protein in tobacco (Nicotiana tabacum). K to M, MOF1-YFP in tobacco. Bars = 5 µm (B–G) and 50 µm (H–M).