Germination analysis of DRT111 knockout mutants, DRT111 OX lines, and drt1111 mutant complemented lines. A to C, Seed germination of drt111 mutants (drt111-1, drt111-2), DRT111 OX lines (35S::FLAG-DRT111 2, 4, and 21), and wild-type (Col-0). Germination percentage of 10 d after-ripened seeds scored in terms of radicle emergence (A) and cotyledon expansion (B) in presence of 0.5 μm ABA and control media (C). Data are means ±sd (n = 150) of five biological replicates. D, Germination percentage of 1 year after-ripened seeds scored in terms of radicle emergence after 3 d in the presence of different concentrations of ABA. E, Germination analysis of drt111 mutants transformed with DRT111 genomic fragment including 1- or 2-kb upstream sequence from the translation start site (1KbDRT111; 2kbDRT111) in pDRT111::DRT111-FLAG constructs. Germination reported as percentage in terms of radicle emergence under control conditions (left) or in the presence of 0.5 μm ABA (middle), and in terms of cotyledon expansion in the presence of 0.5 μm ABA (right). In all germination tests, seeds were stratified for 2 d before incubation at 24°C. The asterisks indicate significant differences compared to Col-0 according to Student’s t-test (^*P ≤ 0.05 and ^****P ≤ 0.0001).