Expression analysis of MADS78 and MADS79. A and B, RT-qPCR analysis of MADS78 and MADS79 in mature pollen and unfertilized ovary (A) and early seed development (48, 72, and 96 HAF; B) relative to leaf tissue from wild-type plants. For statistical analysis, Student’s t test was used, and different lowercase letters indicate significant differences (P < 0.05). Error bars indicate sd (n = 6; two biological and three technical replicates). C, In situ hybridization of MADS78 and MADS79 in mature anthers, unfertilized ovary, and seeds at 48 and 72 HAF. aw, Anther wall; cv, central vacuole; pg, pollen grain. Bars = 0.2 mm.