Derepressed CrMYC2a and ORCA5 synergistically affect TIA pathway genes. Activation of the TDC, STR, LAMT, and SLS promoters, fused to the firefly luciferase (LUC) reporter, by ORCA5, CrMYC2a, and CrMYC2a^D126N in tobacco cells is shown. A reporter plasmid containing the promoter-LUC cassette was coelectroporated into tobacco protoplasts with effector plasmids harboring TF genes. Control (CN) represents reporter plasmid alone. A plasmid containing the GUS reporter, driven by the CaMV 35S promoter and rbcS terminator, was used as an internal control. LUC and GUS activities were measured 20 h after electroporation. The LUC activity was normalized against the GUS activity. Data presented here are means ± sd of three biological replicates. Different letters denote statistical differences as assessed by one-way ANOVA and Tukey’s HSD test: P < 0.05.